Production of Three Monoclonal Antibodies - AOl , B 05 , and Cll - Against B - CeU Leukemia

The advent of MoAb technology has greatly facilitated the identification of various subsets oflymphoid cells [1, 2]. However, most studies have been concentrated on acute lymphoblastic and myelocytic leukemias [14], and relatively few MoAbs that are directed against surface determinants of chronic lymphocytic cells have been reported [5-7]. The reason may be that CLL cell lines are few in number and are not as well characterized as acute lymphocytic or acute myelocytic leukemia cells. In this report we describe the establishment of three hybridomas which secrete MoAbs against an antigen present on malignant B-Iymphocytes in the peripheral blood of most CLL and some non-T, non-B ALL patients. These newly established MoAbs have unique patterns of reactivity to normal and leukemic cells distinct from other reported MoAbs and may be useful for the characterization of certain malignant B cells, either for clinical purposes or for the staging ofleukemic cells.